Description
Principle of the Assay: This kit uses Indirect-Competitive-ELISA as the method. It can detect Chloramphenicol (CAP) in samples, such as aquatic, livestock, honey, milk and other crops/feed, etc. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with CAP. During the reaction, CAP in the samples or standard competes with CAP on the solid phase supporter for sites of CAP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of CAP. The concentration of CAP in the samples can be calculated by comparing the OD of the samples to the standard curve